Nano-Scale Hyperspectral Imaging

CytoViva’s Hyperspectral Imaging Technology was specifically designed to provide quantitative spectral analysis of nanoscale materials (nanoparticles, virus, bacteria, liposomes etc.) imaged with the patented CytoViva darkfield-based microscope technology. This includes the ability to map the presence and location of nanomaterials in a wide range of environments. In addition, hyperspectral imaging is used to characterize unique surface chemistry and functional groups added to nanomaterials. Finally, certain biologicals such as bacteria and pathogens can be spectrally characterized and mapped in tissue and other environments. In all examples, no special fluorescent markers are required.

CytoViva’s hyperspectral imaging capability can also support other standard microscope imaging techniques such as reflected light, fluorescence and transmitted light brightfield. In addition, it can support more macro level image analysis.

A new option is the analysis in the SWIR range (900-1700nm). Typical applications for this are carbon nanotubes and thin films on Si Wafers.

Recently published (February 2016) results in Microscopy Research and Technique have demonstrated the accuracy of CytoViva’s enhanced darkfield hyperspectral microscopy, by comparing its performance against scanning electron microscopy and energy dispersive X-ray spectroscopy (SEM-EDS) and Raman spectroscopy. This work was conducted by the Brenner Research Group at State University of New York (SUNY) Polytechnic Institute, Colleges of Nanoscale Science and Engineering.

See here a Webinar recording with the title: CytoViva Enhanced Darkfield Hyperspectral Microscope Webinar.

A variety of application examples and use instructions for CytoViva can be found here.


Meet users in the user forum and exchange your experience or download useful information.


Application examples:

See below, application examples illustrating some of the ways in which researchers around the world are currently using CytoViva’s Hyperspectral Imaging Technology.

  1. Optical Observation of Nanoparticles
  2. Mapping AuNPs in Live Epithelial Cells
  3. Characterizing and Mapping Doxorubicin in Live Cancer Cells
  4. Identifying Functional Groups on Nanoparticles Used as Drug Delivery Agents
  5. Characterizing Functional Groups on Carbon Nanotubes
  6. Differentiating Aggregating and Non-Aggregating AgNPs
  7. Differentiating AgNPs and AuNPs in Single Cell Culture
  8. Mapping Carbon Nanotubes in Tissue
  9. Characterizing and Mapping Bacteria in Plant Tissue
  10. Identifying Different Strains of Bacillus
  11. Tracking Gold Nanoparticles in the Circulatory System
  12. Nanoparticle Tracking in Whole animal organisms
  13. Airborne Carbon Nanotube Detection

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