Schaefer Group

The HT-X1™ Plus takes bioimaging to the next level, building on the proven success of the HT-X1™. As the first of its kind in the 2nd-generation Holotomography series, the HT-X1™ revolutionized biomedical research with its high-resolution, 3D imaging and unparalleled stability. Its versatile platform, compatible with various imaging plates and powered by the advanced TomoAnalysis™ software, provided researchers with powerful tools for detailed quantitative analysis and reliable imaging across a wide range of applications. Now, the HT-X1™ Plus propels this pioneering technology even further, offering enhancements designed to meet the ever-evolving demands of biomedical research. Experience clearer, more detailed imaging of multi-layered specimens with improved illumination optics and advanced image reconstruction algorithms.

Equipped with a high-spec camera featuring a 4x larger field of view and significantly reduced acquisition time, the HT-X1™ Plus is perfect for high-throughput phenotypic screening of cells and organoids. Its upgraded correlative imaging capabilities—incorporating an sCMOS-based fluorescence module—enable seamless integration of molecular studies with single-cell-resolution 3D images.

The HT-X1™ Plus extends the reach of Holotomography to an even broader array of challenging specimens, including dense organoids, tissue sections, and fast-moving microorganisms. It is a state-of-the-art Holotomography imaging platform, designed to empower researchers with the precision, efficiency, and reliability needed to drive the future of biological and biomedical discovery.

Holotomography is a technique that utilizes low-intensity light to acquire the refractive index of cells from multiple angles. This technology has emerged as an excellent cellular imaging solution, allowing for high-resolution imaging while maintaining the health of the cells by using low-intensity light sources. Tomocube’s second-generation Holotomography, the Tomocube HT-X1, employs low-coherence light sources, ensuring lower toxicity and noise-free high-resolution image acquisition compared to laser-based methods.

The high-resolution images obtained through this technology enable real-time observation of not only the morphology of living cells but also the shapes of subcellular organelles, such as the nucleus, nucleolus, mitochondria, and lipid droplets. The integrated stage-top incubator provides a stable cultivation environment, enabling prolonged monitoring of sensitive cells like stem cells or organoids.

This innovative system is compatible with various commercial imaging plates and supports imaging up to 96-well plates, making it applicable for high-throughput screening. Utilizing the HT-X1 allows for the acquisition of high-resolution images of cells, microorganisms, organoids, and tissue samples. Additionally, Holotomography analysis software, TomoAnalysis, facilitates obtaining quantitative information based on refractive index, including high-resolution 3D images of cells, as well as volume, area, concentration, and dry mass. Therefore, the use of HT-X1 becomes a crucial tool in understanding biological phenomena.

the field of advanced imaging technology, Tomocube’s very first generation of Holotomography stands out as a symbol of innovation. Leveraging groundbreaking 3D quantitative phase imaging technology, the HT-2H employs diffraction tomography to produce high-resolution 3D holographic images of unlabeled live cells and tissues. This innovative imaging series enables the rapid and straightforward acquisition of real-time, nanoscale images of individual cells, without the need for sample preparation.

By reconstructing a 3D distribution map of the refractive index of biological specimen from multiple 2D holograms, HT-2H provides researchers with essential insights into various cellular and subcellular organelle characteristics, such as volume, shape, cytoplasmic density, surface area, or deformability. Meanwhile, its long-term time-lapse imaging capability enables researchers to observe dynamic changes within samples over extended periods.

Additionally, the HT-2H seamlessly integrates the two complementary modalities of Holotomography and fluorescence imaging, excelling in its ability to offer long-term tracking of specific targets while minimizing unwanted stress to cells. Winner of the Microscopy Today 2019 Innovation Award, the HT-2H facilitates broader access for researchers and clinicians to quantitatively investigate cell pathophysiology and assess the effectiveness of drug interventions.

Tomocube’s TomoAnalysis offers advanced capabilities for the visualization and analysis of refractive index(RI) tomograms down to the cellular and subcellular levels. Through a combination of different segmentation strategies and AI-driven deep learning models, the software provides precise quantification results of important parameters such as volume, surface area, sphericity, aspect ratio, and RI concentration of the regions of interest, facilitating a comprehensive understanding of the specimen properties.

With a range of customizable analysis pipeline procedures designed to address diverse user needs, researchers can refine their multimodal image data, extract meaningful measurements, and conduct in-depth quantitative analyses.